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KEY RESOURCES TABLE

Journal: Cell metabolism

Article Title: NTS Catecholamine Neurons Mediate Hypoglycemic Hunger via Medial Hypothalamic Feeding Pathways

doi: 10.1016/j.cmet.2019.11.016

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Other viruses used: retroAAV-CAG-FLEX-tdTomato (Addgene, 7 3 10 12 vg/mL), rAAV9-CAG-FLEX-ChR2-mCherry (Addgene, 1 3 10 13 vg/mL), pGP-AAV1-syn-FLEX-jGCaMP7s (Addgene, 1 3 10 13 vg/mL), retroAAV-EF1α-DIO-FLPo-WPRE-hGHpA (2 3 10 12 vg/mL), rAAV2-EF1α-fDIO-hM3DGq-mCherry (5 3 10 11 vg/mL), rAAV2-EF1α-fDIO-hM4DGi-mCherry (4 3 10 12 vg/mL) CAG: Cytomegalovirus (CMV) enhancer/chicken beta-actin promoter, syn: synapsin promoter, Efl1a: Human elongation factor-1 alpha promoter, FLEX: Cre-dependent flip-excision switch, fDIO: FLP-recombinase dependent DIO.

Techniques: Virus, Plasmid Preparation, Recombinant, RNA Extraction, cDNA Synthesis, Software